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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two additional splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible results are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is specific.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterised by a dramatic periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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